Acetylcholinesterase engineering for detection of insecticide residues
نویسندگان
چکیده
منابع مشابه
Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant food.
A highly sensitive and rapid food-screening test based on disposable screen-printed biosensors was developed, which is suitable for monitoring infant food. The exposure of infants and children to neurotoxic organophosphates and carbamates is of particular concern because of their higher susceptibility to adverse effects. The European Union has, therefore, set a very low limit for pesticides in ...
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امروزه استفاده از منابع انرژی پراکنده کاربرد وسیعی یافته است . اگر چه این منابع بسیاری از مشکلات شبکه را حل می کنند اما زیاد شدن آنها مسائل فراوانی برای سیستم قدرت به همراه دارد . استفاده از میکروشبکه راه حلی است که علاوه بر استفاده از مزایای منابع انرژی پراکنده برخی از مشکلات ایجاد شده توسط آنها را نیز منتفی می کند . همچنین میکروشبکه ها کیفیت برق و قابلیت اطمینان تامین انرژی مشترکان را افزایش ...
15 صفحه اولA Portable Instrument Based on Acetylcholinesterase Biosensor for the Rapid Detection of Pesticides Residues
In this paper, we integrated acetylcholinesterase (AChE) biosensor and detection system to fabricate pesticides residues portable detection instrument for an on-site use. The AChE biosensor was based on the inhibition of enzyme activity that was monitored by the change of oxidation current of the thiocholine using ordinary three electrodes and an own designed potentiostat. The current signal ge...
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The cholinesterases are serine hydrolases that show no global similarities in sequence with either the trypsin or the subtilisin family of serine proteases. The cholinesterase superfamily includes several esterases with distinct functions and other proteins devoid of the catalytic serine and known esterase activity. To identify the residues involved in catalysis and conferring specificity on th...
متن کاملCholine acetyltransferase and acetylcholinesterase: evidence for essential histidine residues.
ABSTKACT: Choline acetyltransferase (EC 2.3.1.6) catalyzes the biosynthesis of acetylcholine according to the following chemical equation: acetyl coenzyme A + choline + acetylcholine + coenzyme A. Ethoxyformic anhydride inactivates the enzyme prepared from bovine brain. Acetyl coenzyme A and coenzyme A, but not choline or acetylcholine, substantially protect against inactivation. The enzyme is ...
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ژورنال
عنوان ژورنال: Protein Engineering, Design and Selection
سال: 2002
ISSN: 1741-0134,1741-0126
DOI: 10.1093/protein/15.1.43